Quantification of IP-10 levels in the spleen post-MCAO. Immunohistochemical quantification of IP-10 protein levels in the spleens of naïve, sham, 24, 48, 72, and 96 h post-MCAO (n= 4, 3, 7, 3, 3, and 7 respectively) demonstrate IP-10 levels are significantly elevated at 24 h and remain elevated out to 96 h post-MCAO compared to naïve spleens (*p<0.0007; a). Representative micrographs of IP-10 stained spleens from naïve (b), 96 h sham (c), 24 h (d), 48 h (e), 72 h (f), and 96 h (g) post-MCAO. Scale bar = 120μm.