Extended Data Figure 6. Physiological development of the hematopoietic system in the absence of Jmjd3.

a, b, Targeting scheme for the generation of Jmjd3^−/− allele (a) and PCR-based quantification of the wild-type and mutant transcripts (b) using a specific primer set for the 3’ end of Jmjd3 cDNA. c, d, Analysis of the fetal liver for lineage markers (c) as well as bone marrow (d) of recipients for hematopoietic progenitors (lineage⁻, c-kit⁺, sca⁺ (LSK) population) for the Jmjd3^+/+ and Jmjd3^−/− genotypes. Representative plots of three independent experiments are shown. e–g, Analysis of major thymic subsets in Jmjd3^+/+ (n=7) and Jmjd3^−/− (n=7). Schematic representation of FACS analysis performed (e). Relative proportions of major cell populations in the thymi of Jmjd3^+/+ and Jmjd3^−/− background (f). mRNA expression of Jmjd3 gene in different stages of thymic development (g). h, and expression of Notch1 target (like Hes1, n=7) in CD4⁺/CD8⁺ double positive and CD4⁻ CD8⁻CD25⁺ lymphocyte progenitor cells. Representative plots (e) as well as average representation (g, h) of seven independent thymi are shown.