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. Author manuscript; available in PMC: 2014 Oct 26.
Published in final edited form as: Arthritis Rheum. 2012 Oct;64(10):3334–3343. doi: 10.1002/art.34556

Figure 1. CD248 is expressed in human and mouse osteoblasts, but not osteoclasts.

Figure 1

A, Real-time polymerase chain reaction was used to analyze the expression of CD248 in primary cell lysates of osteoblasts, macrophages, and osteoclasts from wild-type mice (left) or osteoblasts, peripheral blood mononuclear cells (PBMCs), and osteoclasts from human subjects (right). Bars show the mean ± SD of 3 samples per group. Inset, CD248 expression was also assessed by Western blotting of human cell lysates of osteoblasts (OB), PBMCs, and osteoclasts (OC), using anti-human CD248 (recognizing the band at 175 kd) with anti–β-actin as control (recognizing the band at 47 kd). B, Tibiae from newborn mice were assessed by confocal microscopy for CD248 (blue), Col1a1 (green fluorescent protein under the control of the Col1a1 promoter; green), and alkaline phosphatase (Alk Phos) enzymatic activity (red); nuclei were stained in grey. The merged image in the left panel shows the colocalization of Col1a1 and CD248 (cyan), colocalization of CD248 and alkaline phosphatase (purple), and colocalization of all 3 markers (white). Bar (left panel) = 100 μm. Arrows indicate examples of cells expressing all 3 markers. BC = bone collar; Tb = trabecular bone.