FIG 1.

Characterization of mice with Efv5-deficient T cells. A, Naive CD4⁺CD62⁺ T cells from control and Etv5^fl/fl CD4-Cre⁺ mice were activated with anti-CD3 and anti-CD28 for 5 days. Nuclear lysates were extracted and immunoblotted for Etv5 and β-actin as a control. B and C, Total cells were isolated from the thymus, spleen, and lymph nodes of control and Etv5 mutant mice and stained for cell-surface markers, with the percentage of positive cells (Fig 1, B) and cell numbers (Fig 1, C) shown. Data are representative of 2 independent experiments with similar results (Fig 1, A and B) or are means ± SEMs of 3 mice per group and representative of 2 independent experiments with similar results (Fig 1, C).