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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: J Allergy Clin Immunol. 2014 Jan 31;134(1):204–214. doi: 10.1016/j.jaci.2013.12.021

FIG 6.

FIG 6

Efv5 is regulated by STAT3-activating cytokines in TH17 cells. Naive wild-type CD4+CD62L+ T cells were cultured under TH17-polarizing conditions. A, Kinetics of Etv5 gene expression during TH17 cell differentiation. B and C, TH17 cells were stimulated with IL-6, IL-23, and IL-12 for 2 hours before gene expression analysis by means of qRT-PCR (Fig 6, B) or protein expression by means of intracellular staining (Fig 6, C). D, Naive control and Sfaf3-deficient CD4+ T cells were activated with anti-CD3 and anti-CD28 in the presence or absence of IL-6 for 48 hours, rested overnight, and restimulated with IL-6, IL-12, or IL-23 for 2 hours before gene expression analysis by using qRT-PCR. E, Schematic of Etv5 promoter-containing STAT3-binding sites. F, Cells prepared as in Fig 6, B, were used for ChIP analysis with STAT3 antibody and IgG as a control. Data are means ± SDs of replicate samples and representative of 3 independent experiments with similar results (Fig 6, A-F). *P < .05.