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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: J Allergy Clin Immunol. 2014 Jan 31;134(1):204–214. doi: 10.1016/j.jaci.2013.12.021

FIG 8.

FIG 8

Etv5 binds the Il17a-Il17f locus in TH17 cells. A, Schematic of the Il17a-Il17f locus containing ETS-binding sites. B and D, Naive CD4+CD62L+ T cells were isolated from control and Etv5 mutant mice and differentiated under TH17 culture conditions. ChIP analyses were performed by using differentiated control TH17 cells to examine transcription factor binding (Fig 8, B) or differentiated control and Etv5-deficient TH17 cells to assess histone modification and the association of histone-modifying enzyme (Fig 8, D) at the Il17a–Il17f locus. C, Luciferase activity in Jurkat T cells transfected with increased concentrations of plasmid encoding Etv5 along with IL17A, IL17F, or NFAT luciferase reporters and then activated for 6 hours with PMA and ionomycin. Data were normalized to control samples. Data are means ± SDs of replicate samples and representative of 3 to 4 independent experiments with similar results. RU, Relative unit.