Fig. 2.

The effect of H₂O₂ on the expression of ELL, MR and 11betaHSD2, and ELL-MR interaction in cultured cardiac fibroblasts. Cardiac fibroblasts were treated with H₂O₂ (1 μmol/L) for 48 h. Cardiac fibroblasts without H₂O₂ were served as Ctl.

A: RT-qPCR was performed to evaluate the mRNA levels of ELL, MR and 11betaHSD2 in the cardiac fibroblasts (n = 5 for each group).

B: WB analysis was performed to evaluate the protein levels of ELL and MR in the cardiac fibroblasts. The images (upper) and the results of densitometry (lower) of WB analysis (n = 4 for each group).

Ctl was arbitrarily set to 1. Data are expressed as mean values ± SD.

*P < 0.05 versus Ctl.

C: Cell lysate of cardiac fibroblasts was subjected to IP with anti-MR antibody. Immunoprecipitates were subsequently analyzed by Western blotting with anti-ELL antibody.

Ctl, control; ELL, elongation factor eleven-nineteen lysine-rich leukemia; IP, immunoprecipitation; RT-qPCR, real-time quantitative reverse transcriptase-PCR; MR, mineralocorticoid receptor; WB, Western blot.