Fig. 4.

[³H]proline incorporation into cardiac fibroblasts after knockdown of ELL under oxidative stress.

A: Cardiac fibroblasts were transfected with siELL (50 nmol/L, n = 3). Cardiac fibroblasts transfected with siNT (50 nmol/L, n = 3) were served as control.

B: Cardiac fibroblasts were transfected with siNT (50 nmol/L) with Cor (300 nmol/L) and/or Epl (1 μmol/L) in the presence of H₂O₂ (1 μmol/L). Cardiac fibroblasts transfected with siNT in the presence of H₂O₂ were served as control. n = 8 for each group.

C: siELL transfected cardiac fibroblasts were treated with Cor (300 nmol/L) and/or Epl (1 μmol/L) in the presence of H₂O₂ (1 μmol/L). siELL transfected cardiac fibroblasts with H₂O₂ alone were served as control. n = 8 for each group.

Control was arbitrarily set to 1. Data are expressed as mean values ± SD.

*P < 0.05 versus control.

†P < 0.05 versus Cor (+) Epl (–) H₂O₂ (+).

#P < 0.05 versus Cor (–) Epl (+) H₂O₂ (+).

Cor, corticosterone; ELL, elongation factor eleven-nineteen lysine-rich leukemia; Epl, eplerenone; siELL, ELL siRNA; siNT, non-targeting control siRNA.