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. 2014 Jul 10;3(7):694–704. doi: 10.1016/j.molmet.2014.07.002

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© 2014 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).

PMC Copyright notice

Response of AgRP DTR animals to ghrelin. (A–F) cfos expression in the arcuate nucleus was mainly colocalized to AgRp neurons after ghrelin injection. (G–I) cfos immunostaining in the arcuate nucleus after ghrelin injection in DTR vs. AgRP DTR animals (DTR 137.5 ± 15.39 vs AgRP DTR 23.33 ± 5.365, p < 0.01). n = 4 in each group (J) Food intake of DTR and AgRP DTR animals after either PBS or ghrelin injection. n ≥ 8 in each group. Scale bar = 50 μm.