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SF1-CB1-KO mice have impaired VMN leptin signaling in response to HFD consumption. (A) Representative staining for phospho-STAT3 (dark brown nuclear dots) in the VMN (dotted line) of male SF1-CB1-WT and SF1-CB1-KO mice fed with HFD for 2 weeks and then treated with leptin (5 mg/kg) or its vehicle; (B) quantification of phospho-STAT3 staining in the VMN (n = 4). Scale bar in (A): 50 μm. Data are represented as mean ± SEM. *P < 0.05.
