Figure 6.

Combined AMPK activation and mechanical stress-stimulation mobilize glucose transport by parallel mechanisms without increasing Ca²⁺ release. A) Representative western blots and quantifications from wildtype and kinase-dead (KD) AMPK expressing soleus and B) extensor digitorum longus (EDL) stimulated with AICAR (2 mM, 40′), passive stretch (50 mN, 15′) or the two combined, ***p < 0.001 vs. control using Tukey's post hoc test. C) 2-deoxyglucose (2DG) transport in wildtype and KD AMPK SOL and EDL, stimulated with AICAR (2 mM for 40′) or stretch (50 mN, 15′) or the two stimuli combined, ***p < 0.001 increase above control using Tukey's post hoc test. ‡p < 0.05 AICAR vs. AICAR + stretch. n = 6. Data are mean ± S.E.M.