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. 1995 Apr 25;92(9):4021–4025. doi: 10.1073/pnas.92.9.4021

Peptide mimicry of the meningococcal group C capsular polysaccharide.

M A Westerink 1, P C Giardina 1, M A Apicella 1, T Kieber-Emmons 1
PMCID: PMC42094  PMID: 7732025

Abstract

Sequence analysis of the variable regions of the heavy and light chains of the anti-idiotypic antibody 6F9, which mimics the meningococcal group C capsular polysaccharide (MCP), was performed. The immunogenic site on 6F9 responsible for inducing an anti-MCP antibody response was determined by means of sequence and computer model analysis of these data. Complementarity-determining region 3 (CDR3) was found to be unique in that the sequence tract YRY was exposed on the surface. A synthetic peptide spanning the CDR3 domain was synthesized and complexed to proteosomes (meningococcal group B outer membrane protein). Immunizations of BALB/c mice with the peptide-proteosome complex resulted in a significant anti-MCP antibody response. Immunized mice were protected against infection with a lethal dose of Neisseria meningitidis serogroup C.

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Selected References

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