FIG. 5.

Estimates for rates and estimation qualities from passive-mode single-molecule force-probe experiments of apo-myoglobin. All panels report the estimation results, showing the direct Markov model estimate (black line), a fit to the fluctuation autocorrelation function using a dividing surface at the histogram maximum (minimum between two maxima for filtering) o = 4.6 pN (green line), and spectral estimation (red line). (1a),(1b) The stationary probability of observing a given force value (solid black line). The partial probabilities of states A (gray) and B (orange) obtained by spectral estimation show that there is very little overlap between the states. (2a),(2b) The estimation quality α_o, coinciding with the OQ α̂_o, for spectral estimation. (3a),(3b) Estimated relaxation rate κ₂. (4a),(4b) Estimated microscopic transition rates k_AB (dashed line) and k_BA (solid line).