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. 2014 Sep;5(9-10):320–336. doi: 10.18632/genesandcancer.30

Fig. 5. Effect of TMEPAI on PTEN, Akt, pAkt and CDKIs expression.

Fig. 5

A. Relative expression determined by Western blotting of PTEN, phosphorylated Akt (pAkt), total Akt, p21, p27, TMEPAI and GAPDH in MDA-MB-231 cells expressing control (CON shRNA) or TMEPAI shRNA without and with TGF-β (2 ng/ml) treatment. B) Western blots of HMEC expressing control vector (pcDNA) or human TMEPAI (pTMEPAI) in the absence or presence of TGF-β. C) Relative levels of PTEN and TMEPAI in PTEN null 786-O human renal carcinoma cells expressing doxycycline (DOX) inducible PTEN vector in the absence or presence of TGF-β (2 ng/ml) and/or Doxycycline (1μg/ml). D) Relative expression of PTEN protein in mouse proximal tubule cells (BUMPT) expressing pcDNA or mouse TMEPAI (pTMEPAI) without or with TGF-β (2 ng/ml). TMEPAI is detected with anti-His antibody. E) Growth curves of MDA-MB-231 cells in the absence or presence of TGF-β (2 ng/ml) and LY294002(10μM) alone or together.