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. 2014 Sep;5(9-10):320–336. doi: 10.18632/genesandcancer.30

Fig. 7. Downregulation of PTEN levels by TMEPAI.

Fig. 7

A) Immunoprecipitation by α-TMEPAI mouse monoclonal antibody of MDA-MB-231 cell lysates treated without or with TGF-β and analyzed for TMEPAI, NEDD4 and PTEN by western blotting. Mouse IgG was used as control. B) Immunoprecipitation by α--NEDD4 rabbit polyclonal antibody of MDA-MB-231 cell lysates treated without or with TGF-β and analyzed for TMEPAI, NEDD4 and PTEN by western blotting. Rabbit IgG was used as control. C). Following PTEN induction by DOX (1μg/ml) in 786-O cells for 24 h, DOX was removed and cells were transfected with pcDNA, wild type (wt) or PY mutant of TMEPAI expression vectors. Cells were harvested at 0, 24h and 48h time points and analyzed for PTEN, V5-tag (TMEPAI) and GAPDH by Western blotting. D). Mechanistic model of enhanced PTEN turnover by TMEPAI and NEDD4 in cancer cells.