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. 2014 Sep;5(9-10):320–336. doi: 10.18632/genesandcancer.30

Fig. 9. Role of TMEPAI-PTEN axis in growth and metastatic behavior of breast cancer cells.

Fig. 9

A). Wild type PTEN expression in MDA-MB-231 cells (express control shRNA) inhibits their growth. B). mutant PTEN ((C124S or G129E) expression in growth inhibited TMEPAI knockdown cells (MDA-MB-231 cells expressing TMEPAI shRNA) promotes their growth. C) Mutant PTEN (C124S or G129E) expression alters the F-Actin distribution in TMEPAI knockdown cells (231-TMKD). F-actin was labeled with phalloidin (green) and nuclei were stained with DAPI (blue). Cells infected with wild type PTEN (not shown) had similar actin staining as empty virus (EV). Scale bar: 20μ. D. Representative dorsal bioluminescence images of mice taken at day 35 following intravenous injection of MDA-MB-231-GFP- Luc cells stably expressing control shRNA (CON-shRNA) or TMEPAI shRNA showing lung metastasis. E. Histology of lung alveoli from mice injected with MDA-MB-231 cells expressing control CON shRNA or TMEPAI shRNA and stained with H&E.