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. Author manuscript; available in PMC: 2014 Oct 27.
Published in final edited form as: Arch Biochem Biophys. 2012 May 22;526(2):107–113. doi: 10.1016/j.abb.2012.05.007

Figure 1. Strategy for selecting cell binding and internalizing phage antibodies.

Figure 1

(A) Deplete the naïve human scFv antibody library by incubating with the control cells; (B) incubate the depleted phage library with the target cell followed by washing the cells to remove the unbound phage and eluting the surface bound phage; alternatively, (C) after the removal of unbound phage, incubate cells at 37°C to allow endocytosis of the receptor and the bound phage followed by stripping off the surface bound phage with low pH buffer, and cell lysis to recover the endocytosed phage from inside the cells; (D) amplify phage antibodies from either B or C; (E) repeat selections for 2-3 rounds; (F) screening of monoclonal phage antibodies.