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. Author manuscript; available in PMC: 2014 Oct 27.
Published in final edited form as: Arch Biochem Biophys. 2012 May 22;526(2):107–113. doi: 10.1016/j.abb.2012.05.007

Figure 3. HER2 and transferrin receptor phage antibodies and scFv are internalized into cells.

Figure 3

Top panel: Confocal microscopy with detection of phage antibodies using anti-pVIII antibody. A. Control phage antibody, binds botulinum neurotoxin. B. F5 anti-HER2 phage antibody selected for cellular endocytosis. C. Anti-transferrin phage antibody selected for cellular endocytosis. D. C6.5 anti-ErbB2 phage antibody selected on recombinant ErbB2 [61].

Bottom panel: Confocal microscopy with detection of scFv using an antibody to a Cterminal peptide tag on the scFv. A. Control scFv, binds botulinum neurotoxin. B. F5 scFv selected for cellular endocytosis. C. Anti-transferrin scFv H7 selected for cellular endocytosis. D. C6.5 anti-ErbB2 scFv selected on recombinant ErbB2 [61]. This figure cited images from reference #33.