Table 2.

Published examples of selection of phage antibody libraries on cells.

Cell type	Antibody library	Selection protocol	Specificity	Reference
Human red blood cels	Human naïve scFv	Cell Binding	Anti-B, D (3), HI, E, Kpb	[29]
Human peripheral leukocytes	Semi-synthetic scFv	Cell Binding	Sub-populations of leukocytes	[28]
Melanoma cells	Melanoma immunized scFv (multivalent)	Cell Binding	Melanoma-specific, tumor-specific, lineage-specific	[27]
CD36/CHO myoblast	Human naïve scFv (Vaughan)	Cell Binding	CD36	[44]
Human breast cancer cell line SKBR3	Human naïve scFv (Sheets, monovalent)	Cell Internalizing	HER2, Transferrin receptor	[33]
Tumor cell line A431 and EGFR/CHO cells	Human naïve scFv (Sheets, monovalent)	Cell Internalizing	EGFR	[32]
Human fetal erythroid cells	Human naïve scFv (Sheets, multivalent)	Cell Binding	Fetal nucleated red blood cells	[47]
Human colorectal cancer cell lines	Colorectal cancer cell immunized Fab	Density gradient centrifugation	Colorectal cancer	[43]
Human tumor cell line 22Rv1, SW1990, HPAFII	Human naïve scFv (Gao, 1999)	Cell Internalizing	Transferrin receptor, Integrein α3β1	[34]
Human prostate Cancer cell lines	Human naïve scFv (Sheets, multivalent)	Cell Internalizing	Prostate cancer cells	[35]
Human Leukemia cell lines	Human naïve Fab (Dyax)	Cell Internalizing	FLT3	[53]
Human tumor sections	Human naïve scFv (Sheets, multivalent)	Laser capture microdissection	Prostate cancer cells	[38]
EGFR/CHO Cells	Human naïve scFv (MRC)	Cell Internalizing	EGFR	[54]
33 tumor cell lines	Human naïve scFv-CL-GFP (Morino)	Cell binding with organic solvent separation	21 Tumor associated antigens	[41]
Mesothelioma cells	Human naïve scFv (Sheets, multivalent)	Cell Internalizing	CD146	[39]
Brain tumor sphere cells	Human naïve scFv (Sheets, multivalent)	Cell Internalizing	Glioblastoma multiforme sphere cells	[37]
Tumor cell line MDAMB231	Human naïve scFv (Sheets, multivalent)	Cell Internalizing	EphA2, CD44	[36]