Skip to main content
. Author manuscript; available in PMC: 2014 Oct 27.
Published in final edited form as: Anal Biochem. 2012 Aug 21;430(2):141–150. doi: 10.1016/j.ab.2012.08.005

Figure 3. Analysis of the purity of BoNT/B and BoNT/E domains by SDS-PAGE and RP-HPLC.

Figure 3

Left panel; A Coomassie-stained SDS-PAGE gel was run to characterize the purity of domains. Molecular weight (MW) markers are at each side of the gel. Lane 1: B-LC-HN-a, lane 2: B-LC-HN-b, lane 3: B-LC-HN-c, lane 4: E-LC-HN-a, lane 5: E-LC-HN-b, lane 6: E-LC-HN-c, lane 7: A-LC-HN-mut. Right panel: Reverse phase high performance liquid chromatography (RP-HPLC) was used to assess domain purity and presence of aggregation. Shown here are three E-LC-HN domain examples: (A) E-LC-HN-a; (B) E-LC-HN-b; (C) E-LC-HN-c.