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. Author manuscript; available in PMC: 2014 Oct 27.
Published in final edited form as: Anal Biochem. 2012 Aug 21;430(2):141–150. doi: 10.1016/j.ab.2012.08.005

Figure 4. Analysis of the purity and immunoreactivity of BoNT/B and E domain by size exclusion high performance liquid chromatography (SE-HPLC).

Figure 4

(A-C). When each domain is mixed with its corresponding mAb, the domain peak disappears, indicating that the domain is near 100% immunoreactive. Both 1:1 complex and 2:1 complexed peaks are present. When the domain is mixed with other non-binding mAbs, no complex formation is observed, indicating binding specificity. Shown here are results for two domains: B-LC-HN-a and E-LC-HN-b. (A) B-LC-HN-a with mAb B-a; (B) B-LC-HN-a with mAb B-b; (C) B-LC-HN-a with mAb B-c; (D) E-LC-HN-b with mAb E-b; (E) E-LC-HN-b with mAb E-a; and (F) E-LC-HN-b with mAb E-c.