Table 1. Quantification of fluorescence intensity and calibration of corresponding number of bacilli in Mtb CDC1551 infected rabbit lung sections.
Time post-infection | Pixels (arbitrary units) | Number of bacteria* | ||
Mean | sem | Mean | sd | |
8 weeks | 797 324.16 | 108 333.23 | 6.38 | 0.87 |
16 weeks | 574 820.59 | 51 193.07 | 4.59 | 0.41 |
20 weeks | 191 863.28 | 24 384.57 | 1.54 | 0.19 |
24 weeks: no treatment | 158 243.41 | 13 699.53 | 1.27 | 0.11 |
26 weeks: no treatment | 143 522.97 | 15 280.84 | 1.15 | 0.12 |
24 weeks: immunosuppressed | 3 609 472.74 | 737 211.79 | 18.44 | 28.53 |
26 weeks: immunosuppressed | 1 490 651.68 | 166 659.92 | 11.92 | 20.85 |
Calculated based on 32 X-Y-Z fields using 1.24±0.46 cm tissue sections.