Table 1.
Mutations in the gyrase and topoisomerase IV genes as determined by DNA sequencing and the nalidixic acid minimum inhibitory concentration (MIC) of their corresponding Salmonella strains.
| Strain | MIC (μg/mL) | gy rA a | gyrB | parC | pa rE a |
|---|---|---|---|---|---|
| SE 109/07 | >256 | D87Y | Silent mutations | None | V521F |
| SE 005/03 | >256 | D87Y | Silent mutations | None | Silent mutations |
| SE 022/03 | >256 | D87Y | Silent mutations | None | Silent mutations |
| SE 026/03 | >256 | D87Y | Silent mutations | None | Silent mutations |
| SE 027/03 | >256 | D87Y | Silent mutations | None | Silent mutations |
| SE 028/03 | >256 | D87Y | Silent mutations | None | Silent mutations |
| SE 032/03 | >256 | D87Y | Silent mutations | None | Silent mutations |
| SE 033/03 | >256 | D87Y | Silent mutations | None | Silent mutations |
| SE 047/04 | >256 | D87Y | Silent mutations | None | Silent mutations |
| SE 054/05 | >256 | D87Y | Silent mutations | None | Silent mutations |
| SE 064/05 | >256 | D87Y | Silent mutations | None | Silent mutations |
| SE 066/05 | >256 | D87Y | Silent mutations | None | Silent mutations |
| SE 067/05 | >256 | D87Y | Silent mutations | None | Silent mutations |
| SE 069/05 | >256 | D87Y | Silent mutations | None | Silent mutations |
| SE 073/05 | >256 | D87Y | Silent mutations | None | Silent mutations |
| SE 075/05 | >256 | D87Y | Silent mutations | None | Silent mutations |
| SE 078/05 | >256 | D87Y | Silent mutations | None | Silent mutations |
| SE 080/05 | >256 | D87Y | Silent mutations | None | Silent mutations |
| SE 081/05 | >256 | D87Y | Silent mutations | None | Silent mutations |
| SE 086/06 | >256 | D87Y | Silent mutations | None | Silent mutations |
| SE 101/07 | >256 | D87Y | Silent mutations | None | Silent mutations |
| SE 104/07 | >256 | D87Y | Silent mutations | None | Silent mutations |
| SE 105/07 | >256 | D87Y | Silent mutations | None | Silent mutations |
| SE 106/07 | >256 | D87Y | Silent mutations | None | Silent mutations |
| SE 107/07 | >256 | D87Y | Silent mutations | None | Silent mutations |
| SE 002/03 | 192 | D87Y | Silent mutations | None | Silent mutations |
| SE 076/05 | 192 | D87Y | Silent mutations | None | Silent mutations |
| SE 004/03 | 128 | D87Y | Silent mutations | None | Silent mutations |
| SE 048/04 | 128 | D87Y | Silent mutations | None | Silent mutations |
| SE 097/06 | 128 | D87Y | Silent mutations | None | Silent mutations |
| SE 074/05 | 96 | D87Y | Silent mutations | None | Silent mutations |
| SE 090/06 | 96 | D87Y | Silent mutations | None | Silent mutations |
| SE 068/05 | 64 | D87Y | Silent mutations | None | Silent mutations |
| SE 102/07 | 64 | D87Y | Silent mutations | None | Silent mutations |
| SE 046/04 | 48 | D87Y | Silent mutations | None | Silent mutations |
| SE 049/04 | 48 | D87Y | Silent mutations | None | Silent mutations |
| SE 084/05 | 24 | D87Y | Silent mutations | None | Silent mutations |
| SE 001/03 | 16 | D87Y | Silent mutations | None | Silent mutations |
| SE 065/05 | 16 | D87Y | Silent mutations | None | Silent mutations |
| STM 032/04 | >256 | D87G | None | None | None |
| SE 089/06 | >256 | D87G | Silent mutations | None | Silent mutations |
| SE 095/06 | >256 | D87G | Silent mutations | None | Silent mutations |
| SE 070/05 | 128 | D87G | Silent mutations | None | Silent mutations |
| SE 050/04 | 64 | D87G | Silent mutations | None | Silent mutations |
| SE 012/03 | >256 | D87N | Silent mutations | None | Silent mutations |
| SE 015/03 | >256 | D87N | Silent mutations | None | Silent mutations |
| SE 013/03 | 96 | D87N | Silent mutations | None | Silent mutations |
| STM 043/05 | >256 | S83F | Silent mutations | Silent mutations | Silent mutations |
| SE 055/05 | >256 | S83F | Silent mutations | None | Silent mutations |
| SE 051/04 | >256 | S83I | Silent mutations | None | Silent mutations |
| SE 052/04 | >256 | S83I | Silent mutations | None | Silent mutations |
| SE 099/06 | >256 | S83Y | Silent mutations | None | Silent mutations |
| STM 018/03 | 24 | None | Silent mutations | None | M438I |
| STM 071/07 | 64 | None | Silent mutations | None | None |
| STM 055/05 | 32 | None | Silent mutations | None | None |
| STM 006/02 | 16 | None | Silent mutations | None | None |
| STM 033/04 | 16 | None | Silent mutations | None | None |
| STM 053/05 | 16 | None | Silent mutations | None | None |
| STM 056/05 | 16 | None | Silent mutations | None | None |
| STM 034/04 | 12 | None | Silent mutations | None | None |
| STM 048/05 | 8 | None | Silent mutations | None | None |
| STM 057/05 | 6 | None | None | None | None |
D, aspartic acid; F, phenylalanine; G, glycine; I, isoleucine; M, methionine; N, asparagine; S, serine; V, valine; Y, tyrosine; “silent mutations” denotes synonymous base substitutions in the QRDR of the target genes and thus does not result in amino acid change; “none” denotes wild-type sequence in the QRDR; that is, base substitution did not take place.
aThe first letter indicates the original amino acid, followed by numbers that indicate the position of the amino acid in respective gene, and the last letter indicates the substituting amino acid. For example, D87Y means tyrosine replaced aspartic acid in position 87 in GyrA subunit.