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PCR primer sequences for HRM analysis of gyrase and topoisomerase IV genes.
| Primer | Sequence (5′-3′)b | Amplicon size (bp) |
|---|---|---|
| gyrA-F | CAATGACTGGAACAAAGCCTA | 164 |
| gyrA-R | AACCGAAGTTACCCTGACCA | |
| gyrB-F | TGTCCGAACTGTACCTGGTG | 198 |
| gyrB-R | ACTCGTCGCGACCGATAC | |
| parC-F | CGTCTATGCGATGTCAGAGC | 219 |
| parC-R | ATCGCCGCGAATGACTTC | |
| parE-F | TACCGCGCAGGATCTTAATC | 193 |
| parE-R | GATCGCCACGGAAATATCAT |
bPrimers designed in this study.
