Figure 2. VPS34-IN1 selectively inhibit class III PI3K.

(A) Lipid kinase profiling of the VPS34-IN1 at 1 μM was carried out against a panel of 19 lipid kinases at the International Centre for Protein Kinase Profiling. (B) Summary of the measurements of VPS34-IN1 for the indicated kinases and IP1 levels in cells (see the Materials and methods section for assay conditions). (C) Lipid kinase profiling of VPS34-IN1 inhibitor was carried out against ProQinase panel of 13 lipid kinases. Summary of IC₅₀ measurements for each kinase is presented in the Table. Abbreviations used for each kinase and assay conditions used are defined at http://www.proqinase.com. Dose–response curves for data marked with an asterisk are presented in Supplementary Figure S3.