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. 2014 Jul 15;44(9):2692–2702. doi: 10.1002/eji.201344245

Figure 6.

Figure 6

Reduced Breg cells in spleens of B/cWKO mice under naive, noninflammatory conditions. (A) Gating based on differential expression of CD21 and CD23 on B220+ B cells is shown for marginal zone B cells (MZ) and T2-MZP Breg cells (Breg). Representative dotplots are shown. (B, C) The number of (C) Breg cells and (C) MZ B cells in C57BL/6, WAS KO, and B/cWKO mice is shown as mean + SEM of n = 12 (C57BL/6), n = 11 (WAS KO), and n = 4 (B/cWKO) mice. (D) IL-10-expressing B cells isolated from spleens after stimulation with LPS for 24 h was assessed by flow cytometry. Top panels show IgG controls and bottom panels show gating for IL-10-producing CD19+ B cells. Representative dot plots are shown. (E)The percentage of IL-10-expressing B cells is shown as mean + SEM of n = 8 (C57BL/6), n = 8 (WAS KO), and n = 4 (B/cWKO) mice pooled from six experiments. Statistical significance determined by Student's t-test.