Figure 5.
(a) Luminometry results from OATP1B1 and OATP1B1-FLAG transfected HEK293T cells. The OATP1B1-FLAG is expressed as a percentage of the OATP1B1 (no epitope) data. Each bar is the mean ± SE of 6 individual experiments with 3 dish replicates. *Statistical increase (P < 0.05) compared to OATP1B1 (no epitope) as determined by an unpaired t-test (Graphpad Prism). (b) Mediated uptake of 0.1 μM [3H]E3S was measured at 37°C for 3 min into HEK293T cells transiently transfected with OATP1B1 and OATP1B1-FLAG constructs. Data are shown as uptake in pmol/mg protein/3 min with the pcDNA vector control uptake subtracted. Each bar represents the mean ± SE of 7 individual experiments with 6-well replicates. Uptake compared to vector only control was statistically significant (P < 0.05, unpaired t-test; Graphpad Prism).