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. 2014 Oct 27;8:340. doi: 10.3389/fncel.2014.00340

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Copyright © 2014 De Jesus, Dos Santos, Ribeiro, Pinheiro, Freire, El-Bachá, Costa and de Fatima Dias Costa.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Percentage of cell viability measured by the MTT assay in rat neuron/glial cell co-cultures treated with 300 IU/mL of IFN-γ and 1.0 mg/mL of LPS and infected with Neospora caninum tachyzoites (ratio cell:parasite 1:1). The results are expressed as the percentage of cell viability observed in different treatment conditions and the respective standard deviation compared with untreated/uninfected control cultures (considered as 100%) 72 h post infection. The results are expressed as the mean of the percentage and the respective standard of eight samples, in three independent experiments. “a” represents a significant statistical difference when compared to untreated/uninfected control cultures; (Two-way ANOVA/Tukey’s Multiple Comparison Test—p < 0.05).