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. 2014 Oct 27;8:340. doi: 10.3389/fncel.2014.00340

Figure 5.

Figure 5

Immunodetection (immunoperoxidase) of tubulin βIII of rat neuron/glial cells co-culture treated with 300 IU/mL of IFN-γ and 1 mg/mL of LPS and infected with Neospora caninum tachyzoites (ratio cell:parasite 1:1), 72 h post infection. Control cells maintained in fresh medium exhibit a basal neurite outgrowth (arrow) (A); a drastic impairment of neurite (arrowhead) was observed in N. caninum infected culture (B) and IFN-γ treated cells (C); neurite length (arrow) in IFN-γ/infected cultures (D); LPS/uninfected and LPS/infected cells did not exhibit changes in neurite lenght (E,F). Scale bar = 50 µm. (G) Measurement and statistical analysis of neurite length (percentage of control) and its respective standard deviation compared to untreated/uninfected cultures (considered as 100%) in three samples in three independent experiments. “a” represents a significant statistical difference between the same treatment group; “b” represents a significant statistical difference when compared to untreated/uninfected control cultures; “c” represents a significant statistical difference when compared to untreated/infected cultures (Two-way ANOVA/Tukey’s Multiple Comparison Test—p < 0.05).