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FISH experiment performed on a pleural effusion from a patient with a HER2-amplified breast cancer. More than 2 copies of chromosome 17 (centromeric probes in green) are seen in cell nuclei, demonstrating the tumoral origin of the isolated cells. HER2 amplification (HER2 probes in red) in these cells is demonstrated by a ratio HER2/centromeric probe > 2.2 (as per current clinical guidelines for HER2 status assessment by FISH38). This image was obtained by a deconvolution algorithm.39
