Skip to main content
. Author manuscript; available in PMC: 2014 Oct 27.
Published in final edited form as: J Am Chem Soc. 2013 Mar 11;135(11):4333–4363. doi: 10.1021/ja3109664

Scheme 21.

Scheme 21

Three parallel bioorthogonal reactions in one solution.a, b

a 50 μM 50, 50 μM 78, and 10 μM 82 in 5 mM phophate pH 7.5 with 25 mM NaCl at 37 °C in the presence of 50 mM each 3-methylbutyraldehyde and trans-cyclooctene and 40 mM cyclopentadiene. HPLC, top: starting material peptides; bottom: crude HPLC chromatogram after 30 minutes. All products were confirmed by isolation, mass spectrometry, and comparison to authentic material synthesized independently.

b RN = Ac-Thr-Tyr-, RC = -Asn-NH2.