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1H NMR spectra (amide region) of the four 4-hydroxyproline Ac-TY-4-hydroxyproline-N-NH2 diastereomers. (a) 2S,4R-hydroxyproline (trans-hydroxyproline, Hyp) (1) (red). (b) 2S,4S-hydroxyproline (cis-hydroxyproline, hyp) (4) (blue); (c) 2R,4S-hydroxyproline (d-trans-hydroxyproline, d-hyp) (125) (magenta); (d) 2R,4R-hydroxyproline (d-cis-hydroxyproline, d-hyp) (126) (cyan). Full NMR spectra and TOCSY-derived amide resonance assignments are in the Supporting Information.
