Figure 4.

PLL cellular ROS unaffected by age or NAC treatment. We analysed the ROS⁻ cell proportion in blood CD44⁺ and CD45R⁺ cells in PLL mice at 2–8 (n = 6) and 16–26 (n = 6) months respectively. There was no age difference in the proportion of ROS⁻ cells (a). We treated PLL mice with 40 mm NAC water for 3 months and measured cellular ROS in blood and BM cells. ROS⁻ cell proportions in blood and BM cells showed no change in response to NAC treatment (b). The proportion of ROS⁻ cells was notably higher in blood CD45R⁺ cells than in BM CD45R⁺ cells without a significant effect of NAC treatment (c). PLL, Peromyscus leucopus linville; ROS, reactive oxygen species; NAC, n-acetylcysteine.