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. 2014 Sep 2;111(40):14466–14471. doi: 10.1073/pnas.1412685111

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Fig. 4. — Ebf2 and MyoD are expressed in distinct cells within the somitic mesoderm. (A) Immunofluorescence staining of tdTomato, GFP (Myf5^Cre), and Ebf2 (red) on adjacent sagittal sections of E10.5 and E11.5 Myf5^Cre; mTmG embryos. (B) Costaining of Ebf2 (red) and MyoD (cyan) on transverse sections of anterior somites of E12.5 Myf5^Cre; mTmG embryos. GFP (Myf5^Cre)/tdTomato double staining is shown (far Left). White dashed boxes show magnified regions 1 and 2. (C) mRNA levels of Ebf2 and MyoD in freshly sorted cells from E12.5, E13.5, and E14.5 embryos. Values are mean ± SD, n = 3 experiments; *P < 0.05, **P < 0.01.