Characterization of miR-1174 and miR-1175 in A. aegypti. (A) Genomic organization of miR-1174/miR-1175. (B) Tissue-specific expression of miR-1174. mg, midgut; FB, fat body; OV, ovary; LO, leftover. (C) In situ hybridizations using a 5′ and 3′ digoxigenin (DIG)-labeled locked nucleic acid-modified DNA oligonucleotide complementary to miR-1174 (1174LNA). (D) miR-1174 level in the whole body after antagomir silencing (Ant-1174) compared with the effect of scrambled control antagomir (Ant-Ct) in female A. aegypti mosquitoes. WT shows miR-1174 level in untreated mosquitoes. qRT-PCR, error bars depict ±SEM.