Effect of miR-1174 silencing in A. aegypti female mosquitoes maintained on 10% sugar solution. (A) A female mosquito with the bloated-abdomen phenotype after miR-1174 antagomir (Ant-1174) injection. (B) Digestive system isolated from a female mosquito with bloated-abdomen phenotype. Note an extremely extended crop. mg, midgut. (C) A female mosquito injected with control antagomir (Ant-Ct). (D) Digestive system from isolated Ant-Ct–treated mosquito. (E) Untreated WT mosquito. (F) Digestive system isolated from a WT mosquito. (G) Percentage of female mosquitoes injected with Ant-1174, Ant-Ct, H2O, or untreated WT displaying the bloated-abdomen phenotype. (H) Weight changes per mosquito 3 d PIJ. (I) Survival of female mosquitoes injected with Ant-1174, Ant-Ct, or untreated WT. Error bars depict ±SEM.