Effect of miR-1174 silencing in blood-fed A. aegypti female mosquitoes. (A and B) Mosquitoes that blood-fed post miR-1174 silencing. (A) Percent of mosquitoes for sucking blood. (B) Body weight post blood-feeding. (C–E) Isolated digestive systems from mosquitoes 1 h after blood-feeding. (C) Untreated WT. (D) Ant-Ct–injected. (E) Ant-1174–injected. mg, midgut. (F) Ovaries isolated from female mosquitoes injected with Ant-Ct, Ant-1174, or untreated WT. (G–I) Isolated digestive systems from mosquitoes 24 h after blood-feeding. (G) Untreated WT. (H) Ant-Ct–injected. (I) Ant-1174–injected. (J) Number of eggs per female mosquito injected with Ant-1174, Ant-Ct, or untreated WT. Error bars depict ±SEM.