Skip to main content
. 2014 Sep 22;111(40):14460–14465. doi: 10.1073/pnas.1416278111

Fig. 4.

Fig. 4.

SHMT is a direct target of miR-1174. (A) miR-1174 directly targets AAEL002510 (SHMT) 3′UTR in in vitro Dual Luciferase Reporter Assay. Data represent the average percentage activity (∆ Fold Activity × 100) of triplicate samples. (B) miR-1174 silencing (Ant-1174) results in an increase of the SHMT transcript level. Injection of Ant-Ct has no effect on SHMT levels. (C) Injection of miR-1174 mimic (Mimic-1174) results in a decrease of the SHMT transcript level, similar to that of SHMT RNAi (dsSHMT). (D) Coinjection of Ant-1174 and dsSHMT results in a lower level of the SHMT transcript (Ant-1174/dsSHMT). Coinjection of Ant-1174 and control dsRNA (dsLuc) does not affect an elevated level of the SHMT transcript caused by miR-1174 silencing (Ant-1174). (B–D) qRT-PCR. Error bars in A–D depict ±SEM.