Rescue of phenotypes caused by miR-1174 silencing in female Aedes mosquitoes. (A) Mosquitoes were treated 12 h posteclosion, maintained on 10% sugar solution for 3 d, and then examined. Increase in weight over that of WT was plotted. (B) Mosquitoes treated as in A were blood-fed, and their weight was measured. Increase in weight was plotted. (C) Number of eggs per female mosquito from experimental treatments in B. Ant-1174, miR-1174 Antagomir; Ant-1174/dsLuc, coinjection of Ant-1174 with control Luciferase dsRNA; Ant-1174/dsSHMT, coinjection of Ant-1174 and dsSHMT; dsSHMT, injection of dsSHMT; and WT is untreated. Error bars in A–C depict ±SEM.