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. 2014 Sep 8;111(40):14613–14618. doi: 10.1073/pnas.1407131111

Fig. 2.

Fig. 2.

Genetic requirements for vasiRNA biogenesis. (A) Relative abundance of unique Arabidopsis small RNAs according to their lengths induced by CMV-∆2b in mutant plants defective for RDR1, RDR6, or both. (B) Venn diagram depicting the proportion of loci that posses a twofold or greater enrichment of vasiRNAs in WT plants after CMV-∆2b infection and are also depleted twofold or more in rdr1 plants (Left) or in rdr6 plants (Right) compared with WT plants after CMV-∆2b infection. (C) Northern blot detection of vasiRNAs derived from LHCB1.3 and the antisense strand of 25S rRNA in WT plants and 17 different mutants after CMV-∆2b infection (+) as well as in WT plants after mock infection (−). The same set of RNA samples were also probed for CMV-∆2b siRNAs, tasiRNA ASRP255, microRNA 167 (miR167), and U6 RNA.