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. 2014 Sep 22;111(40):14571–14576. doi: 10.1073/pnas.1324130111

Fig. 3.

Fig. 3.

Gordonoside F induces insulin release via GPR119 from isolated islets. (A) Rat islets were isolated and incubated in medium supplemented with 2.8 mM or 16.8 mM glucose and indicated compounds for 2 h, and insulin in the supernatants were measured. (B) Gene-targeting strategy in generating GPR119 KO mice. The ORF of GPR119 was replaced by a neomycin cassette via homologous recombination. P1–P6 indicate the primers used to characterize the GPR119 KO animals. (C) Representative PCR analysis of the genomic DNA of embryonic stem (ES) cells with targeted homologous recombination. (D) Representative PCR genotyping of DNA samples from tail clips of WT and GPR119 KO mice. (E) Immunofluorescent analysis of insulin and glucagon in pancreatic sections from 8-wk-old male GPR119 KO mice and age-matched WT litters. (F) Islets isolated from GPR119 KO or WT mice were incubated in medium supplemented with 2.8 mM or 16.8 mM glucose and indicated compounds for 2 h, and insulin in the supernatants was measured. Data are means ± SEM (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 versus vehicle control.