Figure 5.

Human umbilical vein endothelial cells (HUVEC) (a,b) cytoskeleton structure and (c,d) actin connectivity under dynamic shear stress condition in cells in the presence of additives incubated for (a,c) 3 days (Early) and (b,d) 5 days (Late) culture duration, as a function of varying levels of glycated albumin and the nature of the shear stress waveform. All data are reported as mean ± standard error of the mean of four independent experiments. ⁺Significantly different from no added albumin and paired BSA (paired by shear stress and culture duration, three‐way ANOVA; P < 0.05). *Significantly different from Early (paired by shear stress and glycation extent, three‐way ANOVA; P < 0.05). ^#Significantly different from normal shear stress (paired by glycation extent and culture duration, three‐way ANOVA; P < 0.05). AGE, advanced glycation end‐products.