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. 2014 Oct 7;136(42):14678–14681. doi: 10.1021/ja508147s

Figure 2.

Figure 2

S. aureus PBP4 has TP activity in vitro. (a) Schematic of assay to monitor PBP4 activity. The PBP4-activated substrate adduct can be attacked by water or X. Three possible degradation products are yielded: A represents unreacted muropeptide, B is the hydrolysis product, and C is a TP product with Gly2 or d-Ser incorporated. (b) LC/MS extracted ion chromatograms (EICs) of a control reaction without PBP4 (i), a reaction with PBP4 (ii), and reactions containing PBP4 and Gly2 (iii) or d-Ser (iv). (M+2H)/2 ions: A, 485.2; B, 449.6; C(Gly2), 506.7; C(d-Ser), 493.2. See Figure S2a for other Glyx traces.