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. 2014 Oct 7;136(42):15010–15015. doi: 10.1021/ja5079464

Figure 3.

Figure 3

Live-cell imaging confirms APH-mediated, targeted coumarin delivery to nucleolin-expressing cells. We incubated APHs that couple a Cy5-labeled nucleolin aptamer (red) with a polymer scaffold loaded with coumarin (blue) with MCF-7 (top) and MCF-10A (bottom) cells, which express high and low levels of nucleolin, respectively. After 10 min, we washed the cells thoroughly; over the next 4 h, we observed nucleolin-mediated cell-surface binding, APH internalization and cytoplasmic payload release in MCF-7 but not MCF-10A cells. Scale bar = 40 μm.