Figure 4. Identification of key regulatory enzymes (a) and drug testing (b) of the arachidonic acid/eicosanoid metabolism model.

a) Key enzymes or enzyme combinations of the DDC model condition of AJ were reverted to control conditions of this strain to judge the effect on the change of the metabolite state. Black dots indicate enzymes or enzyme combinations that were reverted to control conditions. Red dots indicate those enzyme combination that are able to bring back the DDC-treated state to control conditions. b) In silico drug testing of the model by simulating down-regulation of individual enzyme concentrations as given by their respective expression value by 1/3rd-, 1/6th- and 1/9th of the DDC-treated state of AJ.