Skip to main content
. 2014 Oct 27;9(10):e111006. doi: 10.1371/journal.pone.0111006

Table 2. Kinetic equations and their parameters of the arachidonic acid/eicosanoid metabolism model.

R1: PC → AA Inline graphic Vmax = 20.56 nM2s−1, Km = 2500 nM [BRENDA], Ki = 100 nM, A1 = {PLA2}, A2 = {PERK}, I = [AA] nM, S = [PC] nM
R2: AA → 5-HPETE Inline graphic Vmax = 0.01 nM2s−1, Km = 0.0107 nM, Ki = 8.603 nM, A1 = {ALOX5}, A2 = {ALOX5AP}, I = [5-HPETE] nM, S = [AA] nM
R3: 5-HPETE → Inline graphic S = [5-HPETE], Kcat = 0.0012s−1
R4: 5-HPETE → LTA4 Inline graphic Vmax = 9.7953 nM2s−1, Km = 99.913 nM, Ki = 0.709 nM, A1 = {ALOX5}, A2 = {ALOX5AP}, I = [LTA4] nM, S = [5-HPETE] nM
R5: LTA4 → Inline graphic S = [LTA4] nM, Kcat = 0.0012 s−1
R6: AA → 15-HPETE Inline graphic Vmax = 0.003 nM3s−1, Km = 0.067 nM, Ki = 1.004 nM, A1 = {ALOX15}, I = [15-HPETE] nM, A2 = {PKCD}, A3 = {PSTAT3}, S = [AA] nM
R7: 15-HPETE → 15-HETE Inline graphic Vmax = 1.098 nMs−1, Km = 1.58 nM, Ki = 0.0106 nM, A1 = ALOX15, I = [15-HETE] nM, S = [15-HPETE] nM
R8:15-HETE → Inline graphic S = [15-HETE], Kcat = 0.00127 s−1
R9: AA → PGH2 Inline graphic Vmax = 0.168 nMs−1, Km = 3.876 nM, Ki = 0.013 nM, A = {PTGS1}I = [PGH2], S = [AA] nM
R10: PGH2 → PGD2 Inline graphic Vmax = 0.067 nMs−1, A = {PTGDS}, S = [PGH2]
R11:PGD2 → Inline graphic S = [PGD2] nM, Kcat = 0.052 s−1
R12: AA → Inline graphic S = [AA] nM, Kcat = 0.00096 s−1

A, A1, A2 and A3 are the ratios of gene expression or protein levels of the respective enzymes between DDC-treated vs. control mice. S is the substrate of the corresponding reaction. Squared brackets refer to concentration values and curly braces indicate fold changes of DDC treatment vs. control. The Km value of PLA2 is taken from BRENDA [55], and other parameters of the table were fitted using the AJ mice metabolic concentrations (see results section).