Figure 5. rNadA intracellular distribution is affected by ARF6.

Chang cells were transfected in order to overexpress wild type ARF6 (ARF6-wt) or dominant-negative ARF6 (ARF6-Q67L) and then incubated overnight with 200 µg/ml rNadA at 37°C. Afterwards, cells were fixed, permeabilized and double stained for rNadA (green) and ARF6 (red). Merged images are also shown. Scale bar 10 µm. Images are representative of two independent experiments.