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. 2014 Jun 23;136(28):9814–9817. doi: 10.1021/ja5030657

Table 1. Second-Order Rate Constants (k2/Ks) for Acylation of the Active Site of PBP2a by Nitrocefin and by Ceftaroline and Dissociation Constants for Binding of Ceftaroline to the Allosteric Site of PBP2a.

  nitrocefin ceftaroline
enzymes k2/Ks (M–1 s–1) k2/Ks (M–1 s–1) Kd (μM)
wild-type 220 ± 25 4500 ± 640 20 ± 4
N146K 270 ± 70 9150 ± 1560 20 ± 4
E150K 65 ± 5 820 ± 470 44 ± 6
N146K/E150K 330 ± 60 850 ± 140 30 ± 7
N146K/E150K/H351N 230 ± 30 1400 ± 140 26 ± 6