General Parameters | fully tryptic digestion with up to 2 missed cleavages |
25 ppm precursor ion tolerance | |
1.0 Da fragment ion tolerance | |
Static Modifications | +57.02146 Da on cysteine, carboxyamidomethylation |
+28.03130 Da on lysine and the peptide N-terminus, light dimethylation label | |
Dynamic Modifications | +15.99491 Da on methionine, oxidation |
+6.03766 Da on lysine and the peptide N-terminus, heavy dimethylation label |