| Cellectis Bioresearcha 4–12 weeks |
Basic: 4 weeks, validation
of TALEN with SSA yeast assay. |
| First: 4–9
weeks, confirmed efficiency in human cells
and mouse/rat cell lines upon request; validation by deep sequencing. |
| Premium: 12 weeks, validation in custom cell lines,
and validation
by deep sequencing. |
| Life Technologiesa 2–3 weeks |
Create TALEs of either 17.5 or 23.5 repeats. |
| Binding sequence must be 19 or 25 bp long and the 5′ end must contain a T. |
| Options include a
nuclease (Fok1), activator (VP16), repressor
(KRAB), or a functional domain of choice (MCS). |
| GeneCopoeiaa 2–8 weeks |
Creates TALE nucleases
(Fok1) or activators (VP64). |
| Offers various
levels of validation from basic sequencing (2
weeks) to chromosomal level functional validation (8 weeks). |
| Also offers “safe-harboring” a selected
gene
in the AAVS1 chromosomal location via TALEN mediated homologous recombination. |
| FLASHb 1 day |
TAL library made of 96-mer repeats. |
| Can adopt to high-throughput (automated) or medium
throughput
(hand) synthesis. |
| Voytas Kitc 5–7 days |
Uses type II restriction endonucleases to ligate anywhere from
10.5 to 30.5 repeats. |
| Available through Addgene. |
| ICAd 3–4 days |
Monomers are added
to a growing chain to create the complete
DNA binding domain (DBD). |
| Allows custom creation
of DBD of any length. |
| LICe 3 days |
Library of 2-, 5-, 6-, or 18-mer
repeats used to assemble a
DBD with medium or high-throughput. |
| Under
optimal conditions, additional selection steps can be
eliminated, reducing the total time. |
| fairyTALEf 1 day |
Robotic assisted high-throughput liquid phase assembly. |
| Produces TALE activators, repressors, and nucleases
that target
between 14 and 31 base pairs. |
